Manipulating the mouse embryo : a laboratory manual

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This 4th edition of the "Mouse Manual"-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and rewriting this fourth edition to include new information and protocols on:

· generation of induced pluripotent stem cells
· RNA microinjections
· lentiviral microinjections and infection
· assisted reproduction techniques for sperm and embryo cryopreservation
· isolation, generation, and transplantation of spermatogonial stem cell lines
· in utero electroporation of gene constructs into postimplantation embryos
· vibratome sectioning of live and fixed tissues for imaging thick tissue sections
· whole-mount fluorescent staining methods for three-dimensional visualization

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Detail Information

Series Title

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Call Number

616.02'7333 Beh m

Publisher

New York : Cold Spring Harbor Laboratory Press., 2014

Collation

xxii, 814 p. : ill. : ind. ; 29 cm

Language

English

ISBN/ISSN

9781936113019

Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

4th

Subject(s)

Mice--Embryos--Laboratory manuals.
Mice--Genetic engineering--Laboratory manuals.
Transgenic mice--Laboratory manuals.
Mice as laboratory animals.
MEDICAL / Embryology.

Specific Detail Info

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Statement of Responsibility

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