Antibodies : a laboratory manual

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This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include:

· antibody purification and storage
· engineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis
· extensive labeling techniques
· new immunoblotting protocols
· the latest screening and labeling techniques

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Availability

Detail Information

Series Title

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Call Number

571.9'67 Ant

Publisher

New York : Cold Spring Harbor Laboratory Press., 2014

Collation

xxi, 847 p. : ill. : ind. ; 29 cm

Language

English

ISBN/ISSN

9781936113811

Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

SCIENCE / Life Sciences / Biochemistry.
Immunoglobulins--Laboratory manuals.
Immunochemistry--Laboratory manuals.
SCIENCE / Biotechnology.

Specific Detail Info

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Statement of Responsibility

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