Thesis

Fabrication and Characterization of Keratin Pectin Hydrogel Incorporated with Antibacterial Drug for Wound Healing Application through Ionic Gelation

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"Keratin was found to be a promising alternative material as biomaterial due to several
advantages such as supporting cell attachment, fibroblast growth, and naturally abundance.
Unfortunately, keratin also have fragile and poor mechanical properties that limits its usage. On the
other hand, low methoxyl pectin is widely known as a gelling agent and can be formed into hydrogel
by addition of divalent cation but resulted in a weak hydrogel. While, silver nanoparticle has been
widely known as antibacterial agent to overcome antibacterial resistance cases. Thus, in this study the
combination of keratin and pectin as well as silver nanoparticle was fabricated by addition of calcium
chloride that would have a good physicochemical and antibacterial activity. The hydrogel will be
fabricated in 2 concentration which were 2% and 4% total polymer with 3 different ratios; 1:1, 1:2, and
2:1. Shindai’s method will be used in human hair extraction then followed with determination of
molecular weight using SDS-PAGE, protein and thiol content using BCA assay and Ellman assay,
respectively. Hydrogel then fabricated by the addition of calcium chloride and measured its swelling
and erosion index, antimicrobial activity using Kirby- Bauer method, and drug release studies.
Statistical analysis was done to calculate the data significance. It is shown from the result that keratin
and pectin hydrogel possessed good mechanical properties compare to the material on its own. Higher
incorporation of keratin resulted in lower swelling index and higher erosion index due to lower number
of hydrophilic functional groups. In antimicrobial activity, pectin keratin hydrogel will have a better
antibacterial activity compared to pectin alone, with the higher amount of keratin resulted in better
antibacterial activity. While, the inclusion of silver nanoparticle didn’t show any trend due to factors
that might interfere with swelling, erosion index, and antibacterial activity. Lastly, the increase of
concentration of pectin in drug release of silver nanoparticle resulted in a higher cumulative release.
While, in combination of keratin, total cumulative release of hydrogel will be higher with higher
amount of keratin responsible to the highest cumulative release. Therefore, the fabrication of keratin
and pectin hydrogel exhibited good mechanical properties for wound healing application."

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Availability

Detail Information

Series Title

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Call Number

PHA 21-003

Publisher

Jakarta : i3L, Jakarta., 2021

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Hair Keratin
Ionic Gelation
Low methoxyl Pectin
Hydrogel
Antimicrobial Activity

Specific Detail Info

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Statement of Responsibility

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