Thesis

ANTI-INFLAMMATORY EFFECT OF CALOPHYLLUM INOPHYLLUM SEED EXTRACT ON LPS-INDUCED HACAT CELLS AS ATOPIC DERMATITIS MODEL

---

Calophyllum inophyllum Lin has been used as folk medicine for various health problems which has been demonstrated to be a potent anti-inflammatory agent, including atopic dermatitis. In this study, the anti-inflammatory activity of C. inophyllum seed extract was evaluated in LPS-induced HaCaT cells as an atopic dermatitis model. C. inophyllum seed was extracted with ethanol, followed by separation into methanol and n-hexane fraction. Then, its protective effect was evaluated in LPS-stimulated HaCaT cells, followed by quantification of IL-1α, IL-1β, IL-6, IL-8, TNF-α, TSLP, and COX-2 genes to determine the extent of pro-inflammatory down-regulation. n-hexane fraction at 100 μg/mL showed the highest protection in comparison to ethanol and methanol fraction. Further analysis also demonstrated the downregulation of all pro-inflammatory genes after co-treatment of n-hexane extract and LPS, in comparison to LPS treatment alone. Overall C. inophyllum n-hexane seed extract manifests as a promising anti-inflammatory agent for the treatment of AD.

---

Availability

Detail Information

Series Title

-

Call Number

BM 21-006

Publisher

i3L, Jakarta : i3L, Jakarta., 2021

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Calophyllum inophyllum
Atopic Dermatitis
HaCaT
Lipopolysaccharide
Anti-inflammation

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment