Thesis

In vitro Biocompatibility study of Keratin-Pectin composite hydrogels

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The main purpose of 3D in vitro models is to impersonate the complexity of real tissue organs. An
organoid is a 3D micro-physiological system that can capture the structural and functional
characteristics of real organs. Organoid 3D culture is usually cultured from pluripotent stem cells,
organ or tissue fragments which are often cultured in hydrogels. The composite of hydrogels has
been found to produce an optimal extracellular matrix (ECM) that can support tissue and organ
development. Their hydrophilic nature and their crossed-linked polymer networks take after the
hydrated nature of native ECM (Zhao et al., 2020). Hydrogels made up of polymers combined with
other materials have been found to improve the mechanical strength of the polymer and its
biocompatibility. The compatibility of these materials needs to be tested on cells. There have been
various methods in producing a three-dimensional (3D) environment for cells, however
hydrogel-based polymer via ionic gelation is one of the most simple and common methods in
forming a non-toxic polymer that can serve as a 3D environment for cells. This study aims to test the
compatibility of hydrogel-based polymer made up of keratin which is integrated with another
polymer; pectin. These hydrogels will produce a 3D environment for keratinocyte cells (HaCaT
fibroblast cells (NIH/3T3). The histology and viability of the cells would be analyzed. The morphology
and viability of the cells will determine the quality of the hydrogel when it interacts with fibroblast
cells.

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Availability

Detail Information

Series Title

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Call Number

BM 22-023

Publisher

i3L, Jakarta : i3L, Jakarta., 2021

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Pectin
Hydrogels
Keratin-based hydrogels
Cell cytocompatibility

Specific Detail Info

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Statement of Responsibility

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