Thesis

Identification and Comparison of SARS-CoV-2 Peptides Binding to HLA-A*24:02 and HLA-A*24:07

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LA-A*24:07 is unique to the Indonesian population, dominated by Sundanese and Javanese.
As the Caucasian population handles the most worldwide research, a little study on the HLA-A*24:07
has been undertaken. Recently, the connection of SARS-CoV-2 peptides with HLA, notably HLAA*
24:02, has been investigated. The only distinction between HLA-A*24:02 and HLA-A*24:07 is the
residue position 70. No SARS-COV-2 epitope related to HLA-A*24:07 in IEDB was reported until May
19, 2022. NetMHCPan EL 4.1 and IEDB Immunogenicity web servers were utilized in this study to find
42 immunogenic epitopes strongly interacting with HLA-A*24:07. Four top epitope candidates were
used to examine the presence of glutamine70 of the HLA-A*24:02. The glutamine70 was compared to
histidine70 of the HLA-A*24:02 for binding properties assessment. The assessment showed a lower
affinity of the peptide-HLA-A*24:07 complex. A slightly different complex structure was influenced by
glutamine and histidine's distinct side chains. Molecular docking using HPEPDOCK and CABS-Dock
webserver was carried out along with the stability assessment using CABSFLEX. The 3D visualization
and the non-covalent interaction were done using the Pymol and Protein-ligand interaction profiler
(PLIP). Furthermore, the 2167NYMPYFFTL2176 and 1208QYIKWPWYI1217 peptides become the most
promiscuous epitopes for both HLAs due to their large and aromatic side chain residues that contribute
to higher CD8+ T cell response in the primary infection.

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Availability

Detail Information

Series Title

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Call Number

BM 22-002

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

SARS-COV-2
HLA-A*24:07
Caucasian population
QYIKWPWYI
NYMPYFFTL

Specific Detail Info

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Statement of Responsibility

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