Thesis

The evaluation of anti-aging properties of Serum product on primary human dermal fibroblast cell line through extracellular matrix expression analysis matrix expression analysis

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The skin provides conspicuous signs of aging, and many people are willing to spend a grand
amount of money to keep their youthful appearance. Nowadays, treatments to delay the signs of
aging in the skin are available, but most people favor the use of topically applied serum, due to its
high concentration of active ingredients and comfortable finish product. This project highlights the
evaluation of Serum E on primary human dermal fibroblast (HDF), which is a cell line that secretes
extracellular matrix (ECM) of the dermis such as collagen type I, collagen type III, elastin, and
hyaluronic acid. These ECMs serve as excellent biomarkers for skin aging since aged skin experience
reduction of these ECMs. Briefly, the cultured HDF cell line was treated with Serum E, serum base,
and serum active pharmaceutical ingredients (API). The treatments were observed for any cytotoxic
effect using MTS assay and assessed for their anti-aging efficacy through the expression of ECMs that
were measured quantitatively by using enzyme-linked immunosorbent assay (ELISA). Results showed
that Serum E, serum base, and serum are safe to HDF up to concentrations of 4X recommended
usage. The study found that Serum E is able to increase type I collagen secretion to the extracellular
space. However, treatment with Serum E did not stimulate the secretion of type III collagen, elastin,
and hyaluronic acid. Regardless, this study can prove that Serum E contributes to anti-aging effect.
iv

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Availability

Detail Information

Series Title

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Call Number

BM 22-003

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

BM 22-003

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

skin serum
skin treatment
HDF
Anti-Aging

Specific Detail Info

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Statement of Responsibility

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