Thesis

Inflammatory Evaluation of a topical Moisturizer Product on Human Keratinocytes (HaCaT) Cell Line

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Skin barrier disorder is a condition whereby the skin is incapable of preventing the
penetration of exogenous stimuli. As a result, inflammation are also elevated in an effort to eliminate
these materials, resulting in increased sensitivity towards exogenous material. Treating this
dysfunction can be achieved using topical application of prescriptions such as moisturizers that can
tone down the inflammation. However, the moisturizer must first be evaluated for its irritation
potential. Therefore, the aim of this study is to evaluate the inflammatory potential of a topical
moisturizer by measuring the gene expression of pro-inflammatory mediators such as IL1A, IL1B,
CXCL8, and CALCB using RT-qPCR. An MTS assay was performed to determine a non-cytotoxic
concentration of the moisturizer for the gene expression analysis, which were 2.32 mg/mL, 2.11
mg/mL, and 0.21 mg/mL respectively. The gene expression analysis revealed that neither the product,
base, nor API exhibited a statistically significant increase in IL1A, IL1B, CXCL8, and CALCB expression.
However, they were able to decrease the expression of CXCL8 and was statistically significant for the
product and the API. For the CALCB expression, all treatment groups exhibited a statistically
significant decrease in expression, hinting to a possible anti-inflammatory effect of the moisturizer.
Taken together, the moisturizer demonstrated no significant inflammatory response at a
sub-recommended concentration but exhibited an anti-inflammatory capability in the form of CXCL8
and CALCB gene expression downregulation.

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Availability

Detail Information

Series Title

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Call Number

BM 22-006

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Gene Expression
Skin Barrier Disorder
Inflammation
Irritation test
Moisturizer

Specific Detail Info

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Statement of Responsibility

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Other version/related

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