Thesis

Optimization of RT-LAMP Methods for SARS-CoV-2 Viral Load Detection: A Systematic Review

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Prevention and detection of COVID-19 in most countries have been strictly constricted to
detection via qRT-PCR. We need to find better and more efficient methods to detect respiratory viruses
in case another outbreak like SARS-CoV-2 ever happens again. Quick identification and isolation is key to
preventing the spread of respiratory viruses. The more convenient the methods, the more likely we are
able to control the spread. RT-LAMP is a great alternative that can help solve problems such as lack of
resources or time. Saliva sample collection is also a preferable method compared to nasopharyngeal
swab due to the effectiveness and simplicity of it. We would like for this project to be a base study for
further research in creating techniques using RT-LAMP that is cheap, accurate, and high-throughput. Also give an insight as to what is possible with simpler and cheaper methods. The study by Hayden, Kuentzel & Chittur, 2021 showed replicability and high sensitivity (LoD: 0.67 copies/ μL) due to their strategy insaliva sample treatment as well as the multiplexing of genes that they have implemented.

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Availability

Detail Information

Series Title

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Call Number

BM 22-009

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

SARS-COV-2
Covid-19
RT-LAMP
Saliva

Specific Detail Info

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Statement of Responsibility

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Other version/related

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