Thesis

The Evaluation of Moisturizer Effects Towards Skin Barrier Integrity Using Gene Expression Analysis on Human Keratocytes Cell Line

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Skin barrier integrity is mainly maintained at the outermost layer of the skin, which is
cons????tuted by suppor????ve molecules. Ceramide cons????tutes the largest lipids amount in the
intercellular lipid matrix. Filaggrin is a protein involved in the stratum corneum mechanical integrity
maintenance. Hyaluronic acid mainly acts as a humectant to maintain hydra????on. Nowadays,
moisturizers have been developed to enhance skin barrier protec????on. Prior to the usage, it is
necessary to evaluate the efficacy of the moisturizer formula????on. The project aimed to analyze the
moisturizer formula????on effect on skin barrier integrity by measuring the expression of ceramide
biosynthesis, filaggrin, and hyaluronic acid-associated genes. Cytotoxicity tests were conducted prior
to the qPCR analysis. In addi????on, product specificity checks were done to validate the qPCR products.
The cytotoxicity test showed the non-toxic product formula????on. The concentra????on of 2.32, 2.11, and
0.21 mg/mL of product, base, and API were applied due to the low toxicity. The qPCR results showed
elevated FLG expression, but no changes for CERS3, SMPD1, and HAS2 expressions a????er cell
treatment, which indicated the presence of filaggrin produc????on eleva????on, but no changes towards
the ceramide and hyaluronic acid biosynthesis. The product specificity checks revealed the specific
qPCR products, except for SMPD1, in which the difference in sizes and the genera????on of two peaks in
the mel????ng curve analysis proved the low specificity. Nonetheless, the gene expression results
indicate the poten????al of the moisturizer to be further developed to enhance skin barrier
maintenance.

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Availability

Detail Information

Series Title

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Call Number

BM 22-015

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Gene Expression
Moisturizer
Skin Barrier Integrity
Ceramide
Filaggrin
Hyaluronic Acid

Specific Detail Info

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Statement of Responsibility

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