Thesis

Keratin-Pectin Hydrogels as a Bioink Candidate for Cancer Organoid Models

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The low approval rate of anticancer drugs in the cancer industry is due to the inaccuracy
of preclinical models to clinical trials. Models such as 3D in vitro models have become a viable
option since it is more customizable, especially since they can be 3D bioprinted. The use of
biomaterials in 3D bioprinting has become an exciting alternative to tissue engineering and
regenerative medicine. Biomaterials used in 3D bioprinting are used to create stable structures
with increased biocompatibility, biodegradability, and enhanced supply while reducing
immunogenicity and toxicity to use as different scaffolds, including 3D cancer models. While
keratin and pectin are two biomaterials that have been used separately, they have never been
used in combination as scaffolds for 3D cancer models. In this study, keratin and pectin were
combined, made into hydrogels, were assessed for stable mechanical properties such as swelling,
erosion endurance, self-healing abilities, and injectability. Cancer cell lines were also
encapsulated in the keratin-pectin hydrogels to evaluate the cytocompatibility. The hydrogels
were found to be a suitable candidate for a bioink, showing excellent water retention, and the
ability to self-heal and undergo extrusion, the keratin pectin hydrogels also demonstrated
cytocompatibility through the successful encapsulation of HT29 and HeLa cell lines. The
keratin-pectin hydrogels have strong potential to become a preclinical cancer model, however,
further optimization and standardization are needed.

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Availability

Detail Information

Series Title

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Call Number

BM 22-023

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Pectin
Keratin
Hydrogel
bioink
cancer model
cell encapsulation

Specific Detail Info

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Statement of Responsibility

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