Thesis

In vitro Cytoprotective Activity Study of Cocoa Pod Husk (Theobroma cacao L.) Extract against Blue Light

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In recent studies, blue light has been researched for its negative effect in regards to skin
damage and early aging. Some active ingredients including zinc oxide and iron oxide have been
studied for their protecting effect against blue light irradiation. But, more research has been
dedicated to searching for other ingredients that may have a better performance in protecting
the skin against blue light, as well as having other advantages including the availability and more
economic price. Hence, this research focused on the protection of Cocoa pod
husk (Theobroma
cacao L.) ethanolic extract in HaCaT cells, against blue light irradiation, which activity was known
by measuring the cell viability by conducting MTT assay and cell counting or staining with trypan
blue to the HaCaT cell following the blue light irradiation. Upon characterization, Cocoa pod
husk ethanolic extract had higher total phenolic content, but lower total flavonoid content than
some studies with different extraction methods. In addition, for the antioxidant content,
following the DPPH assay, the cocoa pod husk ethanolic extract showed strong antioxidant
activity, while it was not the case from the results of the FRAP and ABTS assay. In comparison to
the skin protection ability of cocoa pod husk ethanolic extract against blue light irradiation, from
both the MTS assay and the cell counting by trypan blue, the extract was found to be able to
protect the HaCaT cells from blue light irradiation, generally increasing as the extract
concentration increase, although it was not statistically significant.

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Availability

Detail Information

Series Title

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Call Number

PHA 22-004

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

blue light
HaCaT cells

Specific Detail Info

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Statement of Responsibility

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