Thesis

Characteristic Evaluation of Keratin, Pectin, and Hyaluronic Acid Hydrogels As Scaffold For 3T3 Cells In 3D Cell Culture

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A huge gap between organ transplant demand and the availability of the organ donor has become a big problem in the medical field for decades. Hence, there is a definite need to find an alternative method obtaining the desired organ for patients. Tissue engineering could become a solution for this problem. Scaffold specifically is a very important factor in tissue engineering and hydrogel has been used as scaffold due to its unique characteristics that are similar to tissue natural environment. Pectin, keratin, and hyaluronic acid were chosen as the candidate for potential components for hydrogel scaffold. In this experiment, the author was trying to find the best composition of hydrogel scaffold to support the growth of 3T3 cells. Keratin was extracted from human hair using the Shindai method, quantified and characterized by BCA assay and SDS-PAGE. Moreover, the hydrogels were tested for swelling and erosion test, as well as undergoing histological examination and with H&E staining. The result showed that pectin-hyaluronic acid hydrogel groups in both 4% and 6% concentration have great capacity to uptake water, resist erosion, and support the growth of 3T3 cells in 3D cell culture. Furthermore, optimization of histological methods, increasing the ratio of pectin and lowering the ratio of keratin for hydrogel 3D scaffold could be done in the future research.

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Availability

Detail Information

Series Title

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Call Number

BM 22-034

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Pectin
Keratin
Hyaluronic Acid
Hydrogel scaffolding
3D cell culture
tissue engineering

Specific Detail Info

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Statement of Responsibility

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