Thesis

Evaluating the antibacterial and antibiofilm activities of common Indonesian medicinal plant extract against Staphylococcus aureus

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Staphylococcus aureus is a bacteria that has gained significant attention due to the emergence
of antibiotic resistance. Some of the factors that contributed to the development of antibiotic
resistance are associated with the formation of biofilm. Abelmoschus manihot and Tinospora crispa
are recognised to have antimicrobial activity and its expected to have biofilm inhibitory effect. In this
experiment, the ethanolic extract of Abelmoschus manihot leaf and Tinospora crispa stem were
investigated for the antibacterial and biofilm inhibition activity against S. aureus and its biofilm.
In this experiment, the antibacterial was conducted through microdilution to determine MIC
and MBC. The concentration that was used in the antibacterial assay was 16 mg/mL, 8 mg/mL, 4
mg/mL, 2 mg/mL, 1 mg/mL and 0.5 mg/mL. The antibiofilm assay was conducted through a microplate
assay dyed with 1% crystal violet, and the parameter used was the percentage biofilm formation. The
five sub-MIC concentrations from antibacterial were used for antibiofilm assay.
The ethanolic extract of A. manihot leaf and T. crispa stem exerts antimicrobial and antibiofilm
properties against S. aureus and its biofilm. The antimicrobial result reveals MIC and MBC of 2 mg/mL
for ethanolic extract of A. manihot leaf and 4 mg/mL for T. crispa stem. The antibiofilm result reveals
a concentration of 1 mg/mL for A. manihot leaf extract shows the highest biofilm inhibition activity
with 74.1 ± 1,609 % inhibition, and T. crispa stem extract at a concentration of 0.0625 mg/mL shows
the lowest biofilm inhibition activity with 31.0 ± 0,898 % inhibition.

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Detail Information

Series Title

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Call Number

BM 22-036

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

BM 22-036

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Medicinal plants
staphylococcus aureus
antibacterial activity
biofilm
antibiotic resistance
Tinospora crispa
Abelmoschus manihot
bioactive compound
antibiofilm activity

Specific Detail Info

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Statement of Responsibility

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