Thesis
Effect Of Chitosan-Based Films With Commercially Available Pandanus conoideus Oil (Red Fruit) On Shelf Life Of Beef Shank Cuts
With the rising demand of convenient and readily-available food products along with
consumer awareness of detrimental effects of excessive plastic usage and food waste, more interest
has developed in bio-film packaging as a means to assist in decreasing the number of waste
produced, both environmentally and food-wise. The main aim of this study is to observe the
potential prolonging of shelf life of beef shank cuts packaged in chitosan-based film incorporated
with RFO and to evaluate the effects on their physical, chemical, and antimicrobial activity. There has
not been identified any studies which incorporated commercially available RFO into chitosan-based
films during the writing of this manuscript. As such this research is important to evaluate their
potentials as novel applications of RFO and active packaging. The results showed changes on their
physical properties producing a statistically significant thicker film along with higher water vapor
permeability at generally higher RFO concentrations (3-4%). As for their chemical properties, no
significant differences were found for their Radical Scavenging Activity (%) for all RFO concentrations.
However it was found to be significant for their TPCC analysis on the highest concentration (CH + 4%
RFO). Antimicrobial testing of all the RFO concentrations showed a significant difference to positive
control (Chloramphenicol), yet was not significant among the different RFO concentrations. This
study also included shelf life testing which evaluated their pH change. As evaluated, the initial pH of
the beef cuts were higher than the acceptable range based on literature. pH of beef shank treated
with chitosan films with different RFO concentrations managed to keep a constant pH throughout
seven days of storage and the highest RFO concentration (4%) had a significant decline in pH on the
last day of storage. Due to the inconclusive results throughout different properties in this study,
chitosan films incorporated with commercially available RFO only affects the physical properties
however did not provide additional preservation more than chitosan films only provide. Future
research should focus on the extraction process of RFO before incorporation into chitosan to develop
an optimum novel application of RFO packaging.
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