Thesis

Development of Chitosan Film Incorporated with Cubeb Essential Oil (Piper Cubeba) for the Preservation of Raw Beef Shank Cuts

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The susceptibility of meat to quality and safety issues has brought numerous attempts to incorporate
antioxidants and antimicrobial agents into the packaging to improve the shelf stability of meat. The
study aims to develop chitosan film incorporated with Piper Cubeba essential oil (PCO) into chitosan
film to improve the shelf life of beef shank cuts. 2% (w/v) chitosan films with 0-3% PCO were
produced and cast on Petri dishes as the film was analyzed for its physical (thickness, color, and water
vapor permeability), mechanical (tensile strength and elongation at break), chemical (FTIR, DPPH,
and TPCC), and antibacterial properties. Increasing concentrations of PCO in chitosan film results in a
lighter film with green-blueish colors, increased thickness, lowered WVP values, and increased
tensile strength where 2% PCO being the highest. Moreover, PCO greatly improves the phenolic
compound from 0.96 to 9.53 mg GAE/g film, scavenging activity from 12.83% to 71.72%, and
antibacterial properties of chitosan against S. aureus from 17.65 to 22.86 mm inhibition zones.
Furthermore, PCO film is able to reduce the microbial count of beef shank cuts for seven days of
storage at 4°C compared to meat that is unwrapped with film (four days). PCO incorporated chitosan
film possesses the future potential to be used as food packaging to extend the shelf life of beef.
Future research is needed to study on the nanoemulstion for reduction of the strong odor in PCO
films, also future research to conduct analysis to determine the protein (Free thiol group assay and
Carbonyl content assay) and lipid oxidation (TBARS assay) in meat as corresponds to the antioxidant
activity of PCO film. Furthermore, a supplemental study on the application of PCO films to wide
range of food products, specifically on ready-to-eat foods could be performed to evaluate the
sensory properties.

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Availability

Detail Information

Series Title

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Call Number

FT 22- 007

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Antioxidant
Antimicrobial
Chitosan film packaging
Piper cubeba essential oils
beef, storage

Specific Detail Info

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Statement of Responsibility

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Other version/related

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