Thesis

In Vitro Methods to Test Antibacterial Efficacy of a Natural Deodorant on Corynebacterium Tuberculostearicum and Staphylococcus Epidermis

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Deodorants have been developed to control axillary skin microbiota, which contributes
to the production of body odor, as well as mask the odor. They are one of the solutions to
address human body odor as a long-term problem for many people, which affects their social
capability negatively. However, no study that we are aware of has examined the antibacterial
effectiveness of a range of deodorants available in Indonesia in vitro. Common testing is done
through clinical trials on human skin, which many dermatological companies find to be
complicated and time-consuming. We tested the antibacterial efficacy of 2 commercially
available (chemically synthesized and naturally derived) and 1 under clinical trials (naturally
derived) deodorants against 2 dominant axillary bacteria: Corynebacterium
tuberculostearicum and Staphylococcus epidermidis using zone of growth inhibition by spot
platting assay and time-kill assay. Results showed that both spot plating assay and time-kill
assay were able to show effectively and significantly the antimicrobial activity of all
deodorants used in this study against both bacteria. Time-kill assay provided more reliable
results of deodorants’ antibacterial performance compared to spot plating assay because it
allowed better interaction of deodorant and bacterial culture when mixed rather than on agar
surface, especially for deodorants with a viscous consistency. Furthermore, the time-kill assay
showed a clear and enumerable bacterial reduction over time across all deodorants and
bacteria. Having said that, the spot plating assay served its aim to show zone of inhibitions
(ZOIs) indicating antibacterial activity of all deodorants with varying efficacy. As for the
deodorant selection, the naturally derived product that is in clinical trials showed a
significantly large ZOIs, close to 20% gradual C. tuberculostearicum reduction over time, and
eradication of S. epidermidis from hour 0. The chemically synthesized deodorant performed
better in the time-kill assay by eradicating both bacteria since hour 0.

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Availability

Detail Information

Series Title

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Call Number

BT 22-001

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Natural deodorants
chemical deodorants
Corynebacterium tuberculostearicum
Staphylococcus epidermidis
in vitro antimicrobial susceptibility test
spot plating assay
timekill assay

Specific Detail Info

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Statement of Responsibility

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