Thesis
Encapsulation of Red Fruit (Pandanus conoideus Lam) Oil by Co-crystallization with Different Emulsifiers as a Natural Food Colorant
Red fruit oil is a native plant found in Papua Island with a high carotenoid concentration, thus
it is considered a promising source of natural food colorant. However, as it is present in the form of
oil, it is prone to oxidation and hard to dissolve in water. Hence, an encapsulation method such as
co-crystallization is required. Sodium caseinate, WPI, and SPI were used in this study as a processing
aid in co-crystallization with two different emulsifier to oil ratios, which are 1:5 and 1:2. The
co-crystalized RFO is then subjected to three analyses, which include TCC, insolubility index,
qualitative solubility, and color measurement. It was found that co-crystallization is a promising
method as it does not significantly reduce the amount of carotenoid of red fruit. As for the use of
emulsifiers, it was revealed that there were no significant differences between emulsifiers and TCC,
as well as color. However, the utilization of WPI 1:2 was found to have a significantly higher
insolubility index. On the other hand, the qualitative analysis showed that the addition of emulsifiers
is beneficial since it prevents separation when the crystals are dissolved in water. Lastly, since the red
color of the product is mainly attributed to the carotenoids, a correlation analysis between color and
TCC was also performed. The result showed a strong, negative correlation with the L* value and a
strong, positive correlation with a* value. Overall, this experiment concludes that the
co-crystallization method is a promising encapsulation alternative to produce natural food colorant
from RFO, although optimizations might still be necessary.
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