Thesis

In Vitro Methods of Antimicrobial Activity Measurement of Naturally Formulated Deodorant on Staphylococcus hominis and Micrococcus luteus

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Human body odor has been a long-term problem for most people, which affects the
physiological condition of the sufferers due to the social stigma given by society. It is caused by the
mixtures of sweats and skin microbiota located in the axillary area. Staphylococcus hominis and
Micrococcus luteus are some of the skin microbiota found in human skin, and these bacteria are
responsible for producing the pungent component of human body odor. Because of that, deodorant
was produced to control the skin microbiota, as well as mask body odor. For that reason, one of
Indonesia’s dermatology companies has formulated a natural deodorant called “Product E” using
natural ingredients as the main ingredients. The antimicrobial testing was done by 2 in vitro
methods; Spot Plating Assay and Time-Kill assay, and a growth curve is also constructed. Aside from
deodorant from Product E, deodorant from Rexona and The Bath Box are also used for comparison.
The result of Product E on Spot Plating Assay is the most significant among others due to the high
average of ZOI, while in Time Kill Assay, Rexona’s deodorant shows the most significant efficiency due
to a 100% bacterial reduction percentage. To improve this study, adding more data using higher OD
and constructing a standard curve would be beneficial to make the data more accurate and credible.

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Availability

Detail Information

Series Title

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Call Number

BT 22-006

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Deodorant
spot plating assay
Staphylococcus hominis
Micrococcus luteus
In Vitro
Time Kill Assay

Specific Detail Info

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Statement of Responsibility

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Other version/related

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