Thesis

In vitro comparative analysis of ethanolic tamanu (Calophyllum inophyllum) extract and tamanu oil effectiveness on treating Atopic Dermatitis (AD)

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Atopic Dermatitis or AD has been introduced since the early 1800s as the most common type of eczema. The reason for the emergence of this disease is still unclear, but researchers believe that inflammation plays an important role in the development of the disease and its severity. Although many drugs are available to lessen the symptoms of the disease, they are known to exhibit serious side effects and thus the need for novel alternative medicine for AD has been a concern in the medical field. One of the possible alternatives is a natural plant extract of the Tamanu plants, which is believed to be beneficial for various dermal issues and also known to be anti-inflammatory. This study will investigate and compare the effectiveness of ethanolic tamanu extract and pure oil, along with its fraction, to lessen the cell degradation of HaCaT cells that have been induced to mimic Atopic Dermatitis by treating the cells with AD inducer (TNFα+IFNγ). The investigation showed that there was no cytotoxicity produced from both all of the treatment solutions and AD inducer when they were treated alone to the HaCaT cells. When combined, however, the treatment solutions and AD inducer were able to elicit lethal cytotoxic effects, especially when the HaCaT cells were treated with ethanolic extract at the concentration of 200 μg/mL and AD inducer at 10+10 ng/mL. This study concluded that the ethanolic extract of tamanu seeds would not be suitable for AD treatment at the concentration of 200 μg/mL. Furthermore, immunocytokine study and phytochemical identification should be performed in the future to elucidate the interactions between the treatment solution and AD inducer better.

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Availability

Detail Information

Series Title

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Call Number

BM 22-051

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Atopic Dermatitis
MTT
Plant extract
Tamanu seeds oil
Ethanolic extract

Specific Detail Info

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Statement of Responsibility

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