Thesis

Nerve Sedation Effect of a Sensitive Skin Moisturizer towards Human Keratinocyte Cell Line: A Gene Expression Analysis

---

Sensitive skin, which can be indicated by unpleasant feelings to the skin, such as burning, tingling, stinging, and itching, has become a growing concern in the last few decades. This situation is mainly due to the increasing level of air pollution, global warming, and the use of skin products that can trigger and worsen sensitive skin conditions. To tackle this problem, numerous products have been developed to treat and cure sensitive skin. This project aimed to investigate the effect of a sensitive skin moisturizer to human keratinocyte cell line. To achieve this, cell viability study using MTS and gene expression analysis using qRT-PCR were conducted after HaCaT cells were treated with the moisturizer, base, and active pharmaceutical ingredients (API). The safety threshold values obtained from the MTS were used for qRT-PCR, which measured the gene expression level of nociceptors TRPV1 and pro-
inflammatory cytokine TNF-α, both of which are heavily correlated to sensitive skin symptoms when upregulated. The resulted gene expression analysis revealed no significant differences in terms of TRPV1
and TNF-α expression in all treated groups compared to the negative control. These results suggest that although the moisturizer might not alleviate sensitive skin symptoms, no sensitive skin symptoms should be elicited upon moisturizer usage in individuals with sensitive skin.

---

Availability

Detail Information

Series Title

-

Call Number

BM 22-050

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

HaCaT
Inflammation
sensitive skin
nerve sedation
TRPV1
TNF- α

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment