Thesis

Genome Sequencing and T-cell Epitopes Prediction of SARS-CoV-2 Non-structural Protein ORF1ab in Indonesia Circulating Strains

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Nowadays, the various variants of SARS-CoV-2 have been found along with the time. It has
been marked down that the antigenic drift takes a part of those occurrences. In genome mapping,
SARS-CoV-2 has the largest Open Reading Frame (ORF) called ORF1ab which translates 16 different
Non-Structural Protein (NSP) for viral replication. This project aims to predict the conservancy of the
ORF1ab domain in Indonesian population against the ancestor strains, Wuhan-Hu-1 strain in order to
validate host T-cell immunity response. Utilizing the Third-Genome sequencing technology, the
SARS-CoV-2’s Indonesian current circulating strains were sequenced and the AVANA software
processed the comparison of ORF1ab T-cell epitopes between ancestor and circulating strain. By the
end of the study, it was found that the ORF1ab T-cell epitopes have conservancy score above 98%
when compare toward Wuhan-Hu-1 during four months of observation with several validation that
the immunogenic mutated T-cell epitopes still associated with HLA Indonesian alleles.

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Availability

Detail Information

Series Title

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Call Number

BM 22-045

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

SARS-COV-2
ORF1ab
Third-Generation Sequencing
Bioinformatic iv

Specific Detail Info

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Statement of Responsibility

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Other version/related

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