Thesis
Usage of Alkaloid Pangium edule Seed Extract to Overcome Contaminations of Foodborne Pathogenic Bacteria Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Klebsiella pneumoniae
Indonesia is well known for its natural resources and cuisines that are rich in spices. Pangium
edule or widely known as “kluwek” is one of the spices used in many traditional Indonesian cuisines.
Research towards these specific spices, especially P. edule is still lacking, especially towards its potential
use. The currently available research only focused on investigating the antioxidant level and
antimicrobial testing toward specific foodborne pathogens that affect tuna fish balls. This research
focused on the methodology to extract alkaloid components from Pangium edule seed and investigate
the effect towards the growth of foodborne pathogens. The efficacy of Alkaloid extract gathered from
Pangium edule seed was measured through the Kirby-Bauer method. The zone of inhibition made by 2%,
4%, and 8% alkaloid extract concentrations was analyzed and compared to the ciprofloxacin and
chloroform and statistical analysis was performed to check the significance of the result. In conclusion,
the Alkaloid component from P. edule seed is able to exert antimicrobial activity against foodborne
pathogens such as Staphylococcus aureus, Salmonella typhimurium, Escherichia coli, and Klebsiella
pneumoniae. The most effective alkaloid extract was against Klebsiella pneumoniae, and was able to
have a higher result than the positive control. Meanwhile, the other bacteria analyzed in the research
showed the alkaloid extract concentration used correlates with the zone of inhibition made and the
result relatively shows no significance compared to ciprofloxacin treatment.
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