Thesis

The Comparison of Biofilm Production Capacity of Vaccine Serotypes (VT), Non-Vaccine Serotypes (NVT), and Non-Typeable Serotypes (NT) of Streptococcus pneumoniae Using An Optimal Broth Medium

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Streptococcus pneumoniae causes many diseases with a high burden worldwide, such as
pneumonia, especially in developing countries including Indonesia. Moreover, S. pneumoniae has
been reported to produce biofilms that have been linked to antibiotic resistance, stronger immune
evasion, and persistent infection. Thus, in vitro pneumococcal biofilms need to be optimally produced
for further studies. This study indicated a better alternative medium to induce pneumococcal biofilm
than a previously published medium, namely, brain heart infusion (BHI) medium. Moreover, this study
provides valuable insight into the influence of different nutrient conditions on pneumococcal biofilm
production. Broth media of different nutritional conditions and supplementation were used to
optimize pneumococcal biofilm formation. The media used were alkaline peptone water (APW),
Mueller Hinton broth (MHB), Todd-Hewitt broth supplemented with 0.5 % yeast extract (THY), brain
heart infusion broth (BHI), and MHB supplemented with 4.5% lysed sheep blood (LSB). Moreover, the
effect of media supplementation with rabbit serum was also investigated. Biofilm formation of
overnight cells was quantified using the microtiter assay with 0.5% crystal violet at 594 nm. LSB was
found as the optimal broth medium to induce pneumococcal biofilms. Furthermore, medium
supplementation with 16.7% rabbit serum did not give significant results. The LSB was then used to
classify the biofilm production capacity of vaccine serotypes (VT), non-vaccine serotypes (NVT), and
non-typeable serotypes (NT) into strong, moderate, and negative production. The results show that
NT has a higher biofilm formation capacity than both VT and NVT.

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Availability

Detail Information

Series Title

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Call Number

BT 22-019

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

biofilm
Streptococcus pneumoniae
broth media
vaccine serotypes
non-vaccine serotypes
non-typeable serotypes

Specific Detail Info

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Statement of Responsibility

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