Thesis

In vitro Cytoprotective Study of Active Ingredients in Sunscreen Against Blue Light

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Nowadays skin damage has been known to occur not only from ultraviolet exposure but also
from the high energy visible (HEV) light called blue light which has a wavelength range of 400 to 500
nm. In fact, the effect of visible light exposure that can be emitted from any electronic device may
cause hyperpigmentation, DNA damage, and oxidative stress that leads to skin aging. With its proven
risk of damaging the skin, some sunscreen products have raised a claim for the photoprotective
capability of their product to protect the skin from exposure to blue light by reflecting and scattering
the light. This increase in claims has occurred alongside the current rise in online activity which
results in advanced usage of blue light-emitting electronic devices and exposure to blue light. Due to
these increasing claims, there is a need to validate them through a scientifically proven and validated
method, such as in vitro assay using immortalized keratinocyte cells called HaCat cells. During the
experiment, the blue light optimization, cytotoxicity, and cytoprotective assay will be done together
with MTS and cell counting assay to determine the result. For the sunscreen product used in this
experiment, it has been indicated that it is cytotoxic when in direct contact with the HaCat cells, thus
can’t provide the cytoprotective ability to the cells. However, it may provide a cytoprotective ability
up to 1.9x and 2.1x in the MTS assay and cell counting method, compared to the internal control with
the concentration of 2 mg/cm2 if the product was not in direct contact with the cells.

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Availability

Detail Information

Series Title

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Call Number

PHA 22-011

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

blue light
HaCaT cells
In Vitro
physical sunscreen
cytoprotectivity
MTS assay
cell counting method iv

Specific Detail Info

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Statement of Responsibility

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Other version/related

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