Thesis

The Effects of Cytokinins (BAP, KIN, and TDZ) on in Vitro Rhizome Multiplication of Rumohra Adiantiformis

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The increase of ornament plants always has a segmented market and unique economical
value. Leatherleaf fern (Rumohra adiantiformis) was one of the examples of ornamental plants and
was widely used in the floristry industry. Plant tissue culture has been an advance and conscientious
alternative approach for plant cultivation to fulfill the commercial and market needs with a very
mindful and continuous process. Throughout the continuous process, this project experiment aimed
to improve the rhizome multiplication of Rumohra adiantiformis in a half-strength MS medium with
the supplement addition of cytokinin plant growth regulators (PGR) with different concentrations
range (BAP 0.5, 1, 2, 4 mg/L, KIN 0.5, 1, 2, 4 mg/L, and TDZ 0.0123, 0.025, 0.05, 0.1 mg/L) by
practicing tissue culture under several parameters. The effectiveness of plant growth, when given
basal media of different strengths of MS medium supplemented with PGR, was to be investigated.
The parameters for data collection analysis include the height of explants, number of newly formed
shoots and roots, rhizomes, and root length parameters. Each treatment was assessed with One-Way
ANOVA and the comparison between MS medium was assessed using Independent t-Test. Cytokinin
regulators, 1 mg/L KIN showed the most significant effect as a plant growth regulator. While the
half-strength MS medium showed the most effective basal media and most suitable media to
promote rhizome multiplication.

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Availability

Detail Information

Series Title

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Call Number

BT 22-022

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Plant tissue culture
in vitro micropropagation
leatherleaf fern
rhizome multiplication
cytokinin plant growth regulators
half-strength MS

Specific Detail Info

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Statement of Responsibility

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