Thesis

Effect of Temperature Shock on Pediococcus Acidilactici: Viability and Molecular Study

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Probiotics are microorganisms that can confer health benefits when consumed in sufficient
amounts. One of the examples of probiotics is Pediococcus acidilactici which has probiotic potential
due to its characteristics, such as its ability to produce pediocin. Probiotics are usually subjected to
various temperature stresses during their processing and storage, which may lead to undesirable loss
of viability of probiotics. There has been little research about the effect of temperature shock on
Pediococcus acidilactici, especially its molecular responses. This study aims to investigate the effect of
various temperature shocks on the viability and stress-related gene expression of Pediococcus
acidilactici. The study was conducted by investigating the viability of P.acidilactici after temperature
shock of -80°C, 4°C, 60°C, 75°C, and 90°C for 5 minutes with 30°C as the control. Gene expression
analysis by RT-qPCR was also conducted to see the expression of the groEL gene in selected
temperature shock treatment, using Ldh as a reference gene. Overall, there was no significant
reduction of viability of P. acidilactici after temperature shock for 5 minutes at -80°C, 4°C, and 60°C in
comparison to 30°C (Log 9.2-9.3 CFU/mL), while there was significant reduction to the culture
subjected to 75°C for (Log 6.17) and 90°C for (0). Gene expression analysis indicated no significant
differences in groEL gene expression of culture subjected to -80°C, 4°C, and 60°C in comparison to
control of 30°C, although the validity of the result is in question due to several possible problems,
mainly gDNA contamination.

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Availability

Detail Information

Series Title

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Call Number

BT 22-022

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

RT-qPCR
Pediococcus acidilactici
Temperature shock
Viability

Specific Detail Info

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Statement of Responsibility

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