Internship Report

Extract Standardization and Oral Acute Toxicity Study of ADAS (Foeniculum vulgare) Extract on Mice as a Potential Covid-19 Treatment

---

Coronavirus Disease-19 (COVID-19) has been an emerging pandemic ever since it arose in
late 2019. Flavonoid, a bioactive compound, was mentioned to be able to treat COVID-19. It acts at
various stages of viral infection, thus inhibiting viral growth. Adas (Foeniculum vulgarae) is a
medicinal plant that is abundant in Indonesia. Adas has been mentioned to be a possible treatment
for COVID-19 treatment due to its flavonoid content. In order to ensure its quality and safety as a
potential medication, the present study performed a standardization and oral acute toxicity study of
adas extract in mice. The standardization and toxicity study was carried out following the Indonesian
Ministry of Health and Organisation for Economic Co-operation and Development (OECD) guidelines,
respectively. The findings suggested that the extract fulfilled almost all quality requirements
mentioned in Indonesian Herbal Pharmacopeia II. Additionally, based on Globally Harmonized
System (GHS) hazard classification, adas was classified into category 4. The present study used doses
of 300 mg/kg body weight (BW) and 2000 mg/kg BW and observed behavioral and weight change,
toxicity symptoms, as well as gross pathology. The extract manifested evident toxicity at the dose of
2000 mg/kg BW in a form of stress, inflammation, walking and grooming behavior, weight loss, and
alterations in the appearance of some organs. The organ aberration was observed through gross
necropsy, where the organs affected was the mice’s lung.

---

Availability

Detail Information

Series Title

-

Call Number

PHA 22-001

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

mice
Adas
Foeniculum vulgarae
acute toxicity

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment