Internship Report
Phytochemical Characterization of Marchantia paleacea, Pogonatum neesii, and Litsea Oppositifolia Ethanolic Extract
i3L is a life science higher education and research institute in Jakarta, Indonesia. Their
Pharmacy department in the School for Life Sciences provides research project opportunities for
students. This project aims to do phytochemical characterization of Marchantia paleacea, Pogonatum
neesii, and Litsea oppositifolia extracts as a preliminary study for two bigger projects that aims to
investigate anti-cancer and anti-aging properties of these extracts. To achieve this aim, phytochemical
screening, assay, and LC-MS analysis was conducted. The phytochemical screening and assay shows
the presence of flavonoid in Marchantia paleacea and Litsea oppositifolia extract, phenolic content in
all three extracts, and a small concentration of alkaloids in all extracts. No extract showed the
presence of saponins. Out of the three quantified functional groups, phenolics were found to be the
most prominent secondary metabolite in all three extracts. LC-MS analysis revealed the presence of
eugenol in Litsea oppositifolia extract. Further analysis using standards or NMR analysis could be
done to further elucidate the components contained in the extracts.
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