Internship Report

Antibiotic Resistant Profiling From Clinical Samples Taken From Primary Clinics in Klang Valley, Malaysia

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Antibiotic resistance is currently reaching alarmingly high levels throughout the world. The discovery of
novel antibiotic resistance mechanisms threatens our ability to treat contagious diseases. Antibiotic
resistance is a severe issue for doctors who treat infectious diseases because it is associated with
increased morbidity and mortality rates. The urgency also occurs in the primary clinic in Klang Valley,
Malaysia. Some patients are displaying clinical signs of resistance to the prescribed drug. Clinical
evidence of the resistance was the continued growth of the infection after therapy, which was indicated
by a protracted sickness and no improvement in signs and symptoms after treatment with conventional
antibiotics. Thus the profiling of antibiotic-resistant genes from each participant's sample will be the
main focus of this investigation. PCR from the throat swab sample is conducted to validate antibiotic
resistance by detecting the presence of antibiotic-resistant genes in bacterial samples. The presence of
the ermC, gyrA, gyrB, grlA, grlB, and tetA genes—which result in resistance to specific antibiotics—was
confirmed by the PCR result. However, all samples lacked the ermB and tetK genes. So, it is evident that
the ermB and tetK genes do not bring about the resistance effect in these situations. In addition, because
the primers for the genes ermA, mrsA, tetM, blaAmpC, tetB, tetC, and tetD are not explicitly bound to the
region of the genes, the thus unspecific result was obtained.

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Detail Information

Series Title

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Call Number

BM 22-004

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

antibiotic resistance
PCR

Specific Detail Info

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Statement of Responsibility

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