Internship Report

In Vitro Cytoprotective Study of Litsea oppositifolia Stem Extract Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells

---

Skin aging is an inevitable natural process in which the skin undergoes changes due to exogenous
factors (UV, pollutants, and toxins) and endogenous factors (genetic mutation, metabolism, and
hormones). Due to the nature of the process, the demand for anti-aging cosmetic products has
increased, rendering the need for alternatives to develop more anti-ageing products. One factor that
promotes aging is the generation of reactive oxygen species (ROS), which can result in oxidative
stress. To counteract this phenomenon, antioxidant products can be used. Botanical plants are rich
sources of antioxidants. One of the plants that exert antioxidant activity is the Litsea genus, whereby
several species from this genus have been recognized for their antioxidant effect. In this study, Litsea
oppositifolia stem extract, which is a species of Litsea genus that has not been studied for its
antioxidant property. Therefore, this experiment aims to study the protective effects of the Litsea
oppositifolia stem extract on HaCaT cells against hydrogen peroxide-induced oxidative stress. First,
cytotoxicity assay was performed on the extract to determine the toxic concentrations of the
compounds, followed by cell viability measurements via MTT assay. A cytoprotective assay was also
performed to test the protective capability of the extract against hydrogen peroxide-induced
damage, wherein the cell viability of the samples was also measured with MTT assay. In this
experiment, it was discovered that the Litsea oppositifolia stem extract showed protective effects at
concentrations of 12.5 ppm and 25 ppm and slight protective ability at the concentration 50 ppm.
The findings from this experiment suggested that the Litsea oppositifolia stem extract can be used as
a natural source of antioxidant.

---

Availability

Detail Information

Series Title

-

Call Number

BM 22-030

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

skin aging
Litsea oppositifolia

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment