Internship Report

Developing CRISPR Strategies to Generate Stable Cell Lines that Expressed GABRA1 Subunit for Alzheimer Disease Models

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GABA or gamma-aminobutyric acid is the major neurotransmitter that acts as inhibitors in
the central nervous system. It is synthesized by the GAD or glutamic acid decarboxylase and
transported into the synaptic system. GABA
receptors are a protein consisting of at least 20 different
subunits and considered as a complex molecule in the human brain. Specifically, the
α1
subunit is
abundantly expressed in the stratum radiatum of the CA1 region as it occupies 60% of the total
subunit present in the brain. It is known that in Alzheimer Disease patients, the GABA
A
α1
receptor
undergoes some alteration, resulting in many harm risks such as losing the binding site for important
drugs as well as the binding of GABA itself which plays a crucial role in preserving memories. The
new technology of CRISPR-Cas9 gene editing is expected to be a promising research for Alzheimer
Disease models as the process is low on the cost, straightforward, and precise. The model is also
chosen because in-vitro model of AD is not yet available. Thus, this in-silico study opens a research
area on in-vitro models of AD especially for developing stable cell lines that can express the GABRA1
subunit. The procedure was done by using CRISPR-Cas9 genome editing technology where knock-in
strategies to initiate homology directed repair is focused. Thus, the project expects that these
procedures can be implemented by hands-on experiments as promising research for in-vitro
Alzheimer ’s Disease models in the future.

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Detail Information

Series Title

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Call Number

BM 22 -032

Publisher

i3L, Jakarta : i3L, Jakarta., 2022

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

GABA
gamma-aminobutyric acid

Specific Detail Info

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Statement of Responsibility

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