Report

Virus Filtration: Minimizing hold-up volume of EPO intermediates product by optimizing assembly design

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Kalbio Global Medika (KGM) are drug manufacturing companies who serve society by
providing high quality and qualified products, such as Hemapo and Efesa. The production goes
through several processes of purification to ensure the safety and quality of the drug. The author was
placed in KGM downstream team and work on one of purification process, virus filtration. Virus
filtration process aim to remove contaminant from the drug by transferring the liquid past the virus
filter with certain arrangement. However, the some arrangement made for virus filter are insufficient
due to high hold-up volume in the assembly. In order to maximize the performance of virus filtration,
hold-up volume study was conducted to identify the most efficient arrangement with the lowest
hold-up volume. The hold-up volume area measured are pressure vessel, post-filter arrangement,
and tubing volume. Hence, the other type of assembly with horizontal virus filter placement is not
applicable since it produces higher hold-up volume present in the virus filter. Therefore, comparison
between arrangement 1 (figure 2) and arrangement 4 (figure 4) was conducted with arrangement 4
as expected as the most efficient arrangement. However, the main problem during the experiment is
that a lot of bubbles were found in the pressure vessel. The air bubble created from collision
between protein liquids traps the bulk protein by adsorption towards the bubble surfaces. During the
experiment, several improvements were found to solve the bubble formation problem and result in
low hold-up volume. The project was successfully done and brought high profit to the company.

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Availability

Detail Information

Series Title

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Call Number

EP BT002

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Kalbio Global Medika
Kalbio Products
Product Process

Specific Detail Info

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Statement of Responsibility

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Other version/related

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