Report

The Effect of Rehydration Media on Growth and Starter Culture Properties of Spray-dried Pediococcus acidilactici

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Pediococcus acidilactici is a lactic acid bacteria that has been found on many fermented
foods around the world, showing its capability as a starter culture. It is gaining research interest due
to its probiotic potential that can provide additional health benefits, further increasing its value as
starter culture. Starter cultures are typically commercialized in powder form, with one of the
common techniques being spray drying. The bacteria within the powder produced, however, is
usually rendered to a dormant state. Hence, rehydration is typically required to reactivate bacteria
prior to its utilization. Although past studies have evaluated the effect of rehydration towards viable
recovery, there is still no further evaluation on its effect towards the growth parameters and starter
culture properties of spray-dried bacteria. Hence, this study aimed to identify the effect of
rehydration and variation of rehydration media towards the growth and starter culture properties of
spray dried starter culture (SDSC). The variation of rehydration media tested included 0.9% NaCl
solution (w/v), type III water, and 20% sucrose solution (w/v). The growth and starter culture
properties (acidification, coagulation, and proteolytic) were evaluated every 3 hours for 30 hours.
Overall, the results showed that rehydration and variation of media used did not cause notable
effects towards growth and starter culture activity. Rehydration step only affected the duration of
adaptation period, but not the maximum growth or starter culture activity. These findings suggested
that rehydration step prior to utilization could shorten the lag period and all three rehydration media
can be used to rehydrate the SDSC powder. Hence, can be adjusted based on the best interest of the
user.

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Availability

Detail Information

Series Title

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Call Number

EP BT003

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

spray drying
Pediococcus acidilactici
starter culture
growth parameters
rehydration

Specific Detail Info

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Statement of Responsibility

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