Report

INVESTIGATING THE EFFECT OF Calophyllum Inophyllum ETHANOLIC EXTRACT ON THE GENE EXPRESSION IN TNF-ɑ AND IFN-γ INDUCED HUMAN KERATINOCYTE SKIN CELLS (HaCaT)

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Atopic dermatitis is a chronic skin disease that affects 20% of children aged three to six
months and 3% of adults worldwide. Despite its prevalence, the exact cause of the disease remains
unclear due to its complexity of the disease. Many available drugs in the market for treating AD focus
on preventing the disease from worsening, such as managing skin infection, hydration, inflammation,
and itch. However, they are known to be inefficient, expensive or cause extreme side effects to the
user; thus, an alternative treatment is needed for AD. The oil from Calophyllum inophyllum or
tamanu has been used as a traditional treatment for various skin-related diseases such as psoriasis,
burn, and acne. In addition, the oil extracted from tamanu seeds has been reported to have several
properties, such as anti-bacterial, anti-fungal, and anti-inflammation. Since AD is related to chronic
inflammation and skin barrier disruption, these properties make tamanu oil an ideal candidate for
AD. Despite this potential, scientific data on the benefits of tamanu oil for AD, especially at the
genetic level, remains scarce. This study investigates the effect of tamanu treatment on the
expression of two skin barrier genes and six inflammatory genes in HaCaT cells induced with
TNF-alpha and IFN-gamma to mimic the condition of AD patients. The investigation shows
upregulation in the treated skin barrier (FLG and IVL) gene expression and downregulation in the
inflammatory (IL-33, MDC, TSLP) gene expression.

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Availability

Detail Information

Series Title

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Call Number

EP BT005

Publisher

i3L, Jakarta : i3L., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Gene Expression
RT-PCR
Atopic Dermatitis
tamanu
Ethanolic extract

Specific Detail Info

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Statement of Responsibility

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