Report

in vitro Cytoprotective Study of Litsea oppositifolia Bark Extract Against UVB Exposure

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Exposure of human skin to the sun has been known to have adverse effects. UV radiation, namely
UVB (280-315 nm), is known to cause sunburn in the skin, as it induces cytokines, neuroactive, and
vasoactive mediators that trigger an inflammatory response. Other than that, UV exposure is also
linked with skin darkening, as it upregulates melanin accumulation in the epidermis. This increases
the risk of melanoma in people. UV exposure also induces reactive oxygen species (ROS) in the skin.
This study compares the Litsea oppositifolia bark extract as a potential solution that protects against
UVB exposure, and its cytoprotective protective abilities were observed towards HaCaT cells. DPPH
assay was performed, where it was observed that LO bark extract had an IC
of 48.660 ppm ± 3.97,
which is weaker than ascorbic acid with an IC
50
50
of 3.853 ppm ± 2.27. The optimum UVB time and
distance deemed cytotoxic to the cells was 6 hours from 15 cm. The extract was not cytotoxic to the
cells, but ascorbic acid is cytotoxic from a concentration of 50 ppm. The cytoprotective assay showed
that LO bark extract exhibited cytoprotective abilities at 100 ppm when exposed to UVB light.
However, AA still had stronger cytoprotective abilities at lower concentrations, namely 25 and 50
ppm. Therefore, it was confirmed that LO bark extract exhibited antioxidant activity and
cytoprotective abilities, although weaker when compared to AA.

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Availability

Detail Information

Series Title

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Call Number

EP BT006

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Litsea oppositifolia
UVB exposure

Specific Detail Info

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Statement of Responsibility

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