Report

IN-SILICO ENHANCEMENT OF CHLAMYDOMONAS REINHARDTII’S ABILITY TO PRODUCE BIOHYDROGEN WITH PROKARYOTIC FHL GENE

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Overusing coal for Indonesian power plants has increased air pollutants yearly. Sustainable
renewable energy is needed; however, most green energies are geographically restricted and
expensive. Hydrogen gas has a high specific energy; thus, it can release more energy. Also, hydrogen
combustion produces water vapor, making it a clean energy source. Nonetheless, mechanical
hydrogen synthesis either results in CO2 release or is expensive. Biohydrogen could fix this issue as
hydrogen synthesis is done through microbial metabolism. Depending on the microorganism
selected, it can reduce the growth price, and yield may be increased. But, the most efficient
biohydrogen production process yields less than mechanical methods. Formate hydrogenlyase can
synthesize hydrogen with formate; thus, it is stipulated that hydrogen synthesis yield increases when
FHL is expressed in a formate-producing organism such as Chlamydomonas reinhardtii. However,
some subunits of FHL are unsuitable for expression in the organism. This in-silico study investigated
the possibility of FHL expression and designing a hybrid FHL complex for C. reinhardtii through FHL
subunit selection, protein parameter determination, identification of signaling peptides, molecular
binding and dynamics, and formation of hybrid FHL complex. The resulting FHL complex has bad
stability through the RMSD test; however, good binding affinity to the substrate and suitable
parameters for expression in C. reinhardtii was detected. Therefore, the protein may be expressed in
C. reinhardtii; however, modifications are required

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Detail Information

Series Title

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Call Number

EP BT014

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

C. reinhardtii
coal
RMSD test

Specific Detail Info

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Statement of Responsibility

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