Report

Investigation on the removal of Depth Filter for an effective Human Recombinant Erythropoietin Production

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Erythropoietin is a glycoprotein hormone produced by the kidney, which triggers the
formation of red blood cells in the bone marrow. However, due to chronic kidney disease, the
erythropoietin hormone is insufficiently produced in anemic conditions. Hence, a therapeutic
recombinant human erythropoietin (rhEPO) is the alternative way to overcome chronic anemia.
Genetically engineered host cells, in this case, CHO cells, are used to culture and express the rhEPO.
However, impurities such as host cell protein (HCP) and host cell DNA (HCD) occur during the cell
culture and must be separated. The objective of this study was to investigate the role of depth filters
regarding HCP and HCD elimination, as well as protein yield and purity. Depth filter is a purification
technique composed of filtration and adsorption processes. Nevertheless, it may bind rhEPO to the
membrane leading to a decrease in rhEPO content. During the downstream process, experiments
were performed by purifying the culture with and without a depth filter. Based on the result, the depth
filter plays a crucial role in removing HCP with a pI of 3.7 to 12. Depth filter is able to bind molecules
with negative and positively charged due to its components. However, for HCD removal, the
experiment showed an insignificant difference between using and not using depth filters. These
results provide new insight into other up-scale downstream processing and performance
characteristics of depth filters containing diatomaceous earth.

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Availability

Detail Information

Series Title

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Call Number

EP BT021

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

depth filtration
purification
erythropoietin
host cell proteins
host cell DNA

Specific Detail Info

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Statement of Responsibility

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