Thesis

The Effect of Primary Drying Temperature on Freeze-dried Mesenchymal Stem Cell Secretome Composition

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Mesenchymal stem cells (MSC) are multipotent stromal cells capable of regenerating and
differentiating into various tissues. MSCs can secrete over 200 different types of these bioactive
molecules called secretomes, that contain chemokines, interleukins, growth factors, and extracellular
matrix proteins. However, the bioactive molecules present in these can degrade over time due to
temperature. This study compares different primary drying temperatures of the freeze-drying
process and cryoprotectant choice towards protein content and purity. Freeze-drying of the
secretome samples using 4% and 8% trehalose v/v was performed, where 8% showed a smaller
decrease in the protein content. Furthermore, the secretome samples were freeze-dried at a primary
drying temperature of -35°C, -30°C, and -25°C. The protein content and purity were then analyzed
through pH measurement, BCA assay, and SDS-PAGE. There were no significant changes in the pH of
the samples at different drying temperatures. In the BCA assay, there was no significant difference in
the secretome decrease in protein as the temperatures were increased. However, SDS-PAGE results
showed an increase in bands as the primary drying temperature. This indicates that although the
proteins are broken down into smaller proteins, the total content was unaffected by the
freeze-drying process.

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Availability

Detail Information

Series Title

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Call Number

BT 23-003

Publisher

: i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

freeze-drying
Mesenchymal stem cells
Secretome
BCA assay
SDS-PAGE

Specific Detail Info

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Statement of Responsibility

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