Thesis

Evaluation of Heat Denaturation and Adsorption-Desorption as a Method for Concentrating Pediocin from Pediococcus acidilactici Fermentation

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A preliminary antimicrobial assay showed the antimicrobial activity of P. acidilactici but the
SDS-PAGE did not reveal pediocin, an antimicrobial peptide produced by Pediococcus strain. The
efficient downstream processing of P. acidilactici fermentation is studied to analyze pediocin. This
research is conducted to assess the compatibility of different methods in concentrating pediocin in
the cell-free supernatant (CFS) i.e. heat denaturation and adsorption-desorption. In heat
denaturation, the thermostable pediocin will remain soluble and can be observed in the supernatant
while removing unconcerned proteins. From five different temperatures, the result of 80°C showed
the highest inhibition zone. Remarkably, heating of CFS at 100°C and 120°C still showed antimicrobial
activity during agar-well diffusion assay against L. monocytogenes with only a slight decrease in
activity; thus, thermostable peptide might be involved. While the Bicinchoninic acid (BCA) assay
showed an irregular pattern indicating that heat does not significantly affect the protein
concentration. In adsorption-desorption, bacteriocin will attach to the cell at a pH of 6.5 and will be
detached when adjusted to 2. The study found no antimicrobial activity from fermentation broth
subjected to adsorption-desorption. Hence, the pediocin failed to be obtained through the
adsorption-desorption and this method is not suitable for the pediocin synthesized by this specific P.
acidilactici strain studied in this research. Future studies could include ammonium sulfate precipitation with optimum saturation percentage to further concentrate pediocin and detection of pediocin with Tricine-SDS-PAGE with 16% gel

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Availability

Detail Information

Series Title

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Call Number

BT 23-005

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Fermentation
Protein
Antimicrobial Activity
Pediococcus acidilactici
pediocin
heat-stable

Specific Detail Info

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Statement of Responsibility

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