Thesis

Bacillus tropicus as Crude Oil Degrading Bacteria: Comparing Planktonic and Immobilized Cells

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Bacillus tropicus is a novel bacteria for hydrocarbon degradation. A previous study
analyzed Bacillus tropicus as a part of a bacterial consortium, but did not divulge into the
specific capabilities of Bacillus tropicus alone. Additionally, the study was performed on
planktonic cells. On the other hand, immobilized cells are regarded to be more efficient
for degradation due to the membrane protection and stability in maintaining cell density
and high-functioning cells. This study investigates Bacillus tropicus as an independent
hydrocarbon degrading bacteria both in planktonic and immobilized forms to compare its
efficiency. The bacteria was immobilized onto a polyvinylidene fluoride (PVDF)/cellulose
triacetate (CTA) membrane where membrane where different concentrations of CTA (%
w/w) were composited and analyzed for its effect on the membrane characterization. It
was found that lower CTA concentrations (< 0.5% w/w) affects the membrane differently
from higher CTA concentrations (> 1% w/w). This study also finds that Bacillus tropicus
are more inclined to attach on membranes that are < 90% porous, and immobilized cells
are more capable of degradation compared to planktonic cells. In conclusion, the study
was able to demonstrate that Bacillus tropicus is capable of degrading crude oil in as an
independent bacteria. The study also provides an insight into bacteria optimization
through bacteria immobilization that was found as more effective in degrading crude oil
than planktonic cells.

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Availability

Detail Information

Series Title

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Call Number

BT 23-007

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Bacillus tropicus
Crude Oil
Hydrocarbon Degradation
PVDF/CTA2
16.3159 mA/m2

Specific Detail Info

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Statement of Responsibility

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