Thesis

Utilization of Gel-filled Microwell Array Device to Isolate Potential Naphthalene-Degrading Bacteria from Sediment Sample

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The isolation and cultivation of indigenous bacteria poses a great importance for examining potential
biodegradation microorganisms for a contaminated site. While the traditional method utilized for the
isolation and cultivation method of bacteria has significant limitations and drawbacks, this study
proposed the usage of a microwell array device that is able to isolate different bacteria at once. The
aim of this study is to isolate diverse bacteria through the utilization of microarray device and further
assess them for their potential naphthalene-degrading abilities. Inoculums were isolated from a
sediment sample from Ardour River, France, through the usage of the microarray device that was
further analyzed for their potential naphthalene degrading abilities by incubating them within ONR7a
medium and naphthalene for 15 days at 30
o
C. The isolated inoculums were further plated onto ONR7a
agar with naphthalene and R2A agar to observe their diversity. A total of 12 inoculums from T1-ext
microdevice and 20 inoculums from T3-ext microdevices were obtained where 1 inoculum (T3A8)
shows a naphthalene degradation of up to 86%. Through plating the inoculums, 11 different colonies
were observed from the inoculums isolated from both T1-ext and T3-ext microdevices, whereby only
6 different colonies were observed from the enrichment cultures. Although further research is still
needed to conclude whether the microdevice serves as a better alternative than traditional methods,
this research still show promising results of the usage of the microdevice for the isolation of bacteria.

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Availability

Detail Information

Series Title

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Call Number

BT 23-017

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

bacteria
Microwell array device
environmental sample
naphthalene
biodegradation

Specific Detail Info

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Statement of Responsibility

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Other version/related

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