Thesis

Immunoinformatics Pipeline for Multi-Epitope Peptide Vaccine Design Based on the Nucleocapsid Phosphoprotein of Indonesian SARS-CoV-2 B.A.2 Isolates

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COVID-19 were identified as viral diseases which was caused by the Severe Acute Respiratory
Syndrome Coronavirus type 2 (SARS-CoV-2). The SARS-CoV-2 are known to be the family of
coronaviridae. As we all know that patients that were infected by the COVID-19 has risen
significantly since the very early outbreak that happens in the early December 2019 specifically at
Wuhan, China. Researchers around the world are trying their best to find the best solution to
solve this pandemic. That’s why in this study, we will try to develop a new methodology in
designing vaccine for viral diseases especially the COVID-19. Therefore, this study aimed to design
a multi-epitope peptide based vaccine which utilizes the Nucleocapsid Phosphoprotein (N-
Protein) of the Indonesian B.A.2 Isolates. 34 SARS-CoV-2 isolates were obtained from the GISAID
Database and NCBI GenBank. These samples were used to examine the evolutionary relationship
of the SARS-CoV-2 through phylogenetic analysis and determine whether these nucleocapsid
proteins are well-conserved. The molecular docking approach is then used to visualize the
molecular interaction of the Toll-Like Receptors with the Vaccine Constructs. Based on the results
that had been acquired during the analysis process, it can be concluded that the Vaccine Construct
(GIGDPVTCLKSGAICHPVFCPRRYKQIGTCGLPGTKCCKKPEAAAKDQVILLNKHIDAYKTAAYLSPRWYFYYA
AYTPSGTWLTYAAYNTASWFTALAAYYYRRATRRIAAYKHWPQIAQFAAYFAPSASAFFGPGPGDQVILLNK
HIDAYKTGPGPGKDQVILLNKHIDAYKGPGPGFKDQVILLNKHIDAYGPGPGNFKDQVILLNKHIDAGPGPGK
DQVILLNKHIDAYKGPGPGFKDQVILLNKHIDAYGPGPGASAFFGMSRIGMEVTEAAAKGIGDPVTCLKSGAI
CHPVFCPRRYKQIGTCGLPGTKCCKKP) that was constructed base on the Isolates from Maluku might
be a potential vaccine construct candidate to develop a Multi-Epitope based Peptide Vaccine.

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Detail Information

Series Title

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Call Number

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Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Bioinformatics
SARS-COV-2
vaccine design
Multi-Epitope
Nucleocapsid Phosphoprotein
COVID- 19

Specific Detail Info

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Statement of Responsibility

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