Thesis

Effect of Spray-drying Towards Pediococcus acidilactici Adherence Ability to Reduce Pathogen Adhesion to The Surface of Intestinal Epithelial Cells

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"Pediococcus acidilactici is a non-pathogenic probiotic bacteria that can improve gut health by
adhering to intestinal cells and inhibiting pathogen adherence. To create an easy-to-use product,
probiotics are often produced in powdered form using spray drying. However, this drying method can
affect the probiotic's viability and functionality. This study examined the impact of spray drying inlet
temperature on P. acidilactici's ability to adhere and inhibit pathogen adherence. The hydrophobicity
of the cells was measured before and after spray drying, found that spray-dried P. acidilactici
maintained high hydrophobicity (96.58-98.92%). The study also evaluated the adherence rate of P.
acidilactici to HT-29 cells before and after spray drying, showing a decrease in adherence rate by
37.68%, 30.38%, and 32.64% (SD120, SD150, SD170) after spray drying. Additionally, P. acidilactici's
ability to inhibit pathogen adherence, specifically Listeria monocytogenes and Staphylococcus aureus,
was assessed by counting pathogen attachment to HT-29 cells in the presence of P. acidilactici.
Without P. acidilactici, S. aureus exhibited a low adherence rate (1.19 ± 0.008%), while L.
monocytogenes adherence rate was 14.34 ± 0.033%. The presence of free-cell P. acidilactici
significantly reduced L. monocytogenes adherence to 88.16 ± 0.07%. However, after spray drying, P.
acidilactici increased L. monocytogenes adherence (ranging from 46.19% to 10.47%) possibly due to
the presence of wall material like gum arabic and whey protein isolate. The inlet temperature during
the spray drying process did not affect the cell surface hydrophobicity of the P. acidilactici,
significantly impacting adherence and pathogen inhibition. Further research is needed to identify the
effect of wall material on adhesion and inhibit pathogen adherence."

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Availability

Detail Information

Series Title

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Call Number

BT 23-024

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

spray drying
ediococcus acidilactici
adherence
pathogen adherence inhibition
cell surface hydrophobicity

Specific Detail Info

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Statement of Responsibility

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