Thesis

Effect of Inoculation Size of Spray Dried Pediococcus Acidilactici on Milk Fermentation

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Fermented dairy has been consumed widely and estimated to have inclining demand due to
health benefits offered. Discoveries and studies on alternatives of lactic acid bacteria (LAB) starter
culture in producing fermented dairy with more health benefits have been carried out to meet the
demand. Pedioccocus acidilactici, gained popularity due to its well-known bacteriocin, Pediocin PA-1
and Pediocin AcH, also cholesterol lowering properties and good fermentation ability. In order to
create a starter culture with lower contamination rate and enhanced shelf life, previous study had
developed spray dried P. acidilactici starter culture. Nevertheless, this product has not yet been
tested on optimum inoculation size to obtain fermentation efficiency and productivity.
This study aimed to assess the effect of inoculation size of spray dried P. acidilactici on milk
fermentation. Three inoculation sizes (1%, 2%, and 4%, w/v) were incorporated into separate
pasteurized milk and were incubated at 45oC overnight. Milk samples with different inoculation size
were investigated for their acidification properties, microbial growth, and syneresis level in 0, 8, 16,
20, and 24 hours incubation time. Milk inoculated with 4% w/v spray dried P. acidilactici, or SC4,
presented higher microbial growth, acidification and lower syneresis in comparison to other samples.
The results confirmed that increasing inoculation size affects the fermentation characteristic, where
higher inoculum size develops higher final viable colony count, faster fermentation rate, titratable
acidity, and reduces the pH and syneresis.

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Detail Information

Series Title

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Call Number

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Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Fermentation
Pediococcus acidilactici
starter culture
inoculation size

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