Thesis
The effect of Plant Concentration of Ultrasound Assisted Ethanolic Clove (Syzygium Aromaticum) Extracts on Antioxidant and Antimicrobial Activity
Syzygium aromaticum, commonly known as cloves, is considered as one of the
richest dietary sources of antioxidant and polyphenolic compounds. The bioactivity and
antimicrobial activity of this spice is enhanced by using novel extraction techniques such as
ultrasound-assisted extraction (UAE). Sample-to-solvent ratio is one of the important factors
that impact the functional properties of plant extracts. Hence, this study aimed to optimize
the sample-to-solvent ratio for ultrasound-assisted ethanolic clove extracts. The resulting
antioxidant properties were measured through 2,2-diphenyl-1-picrylhydrazyl (DPPH), total
phenolic content (TPC), and total flavonoid content (TFC) assays. The antimicrobial
properties were measured using well diffusion technique, minimum inhibitory concentration
(MIC), and minimum bactericidal concentration (MBC) on Escherichia coli and
Staphylococcus aureus. The 2.5% (w/v) plant concentration conferred the highest
antioxidant activity (44.69 ± 3.44 μg/mL, p-value = 0.027) , TPC (90.04 ± 7.47 mg /g, p-value
=
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