Thesis

In-Vivo Study Of Wound Healing And Antimicrobial Properties Of Biocellulose Incorporated With Black Tamanu Oil In DiabeticInduced Mice and Non-Diabetic Rabbits

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Chronic diabec wound is a common complicaon in diabetes mellitus which is caused by a
prolonged wound-healing process from a persistent inflammaon phase. Thus, proper wound
treatment is needed to promote wound healing and protect the wound from infecon. Biocellulose
(BC) as wound dressing provides a moist environment to protect skin and promote cellular migraon
and proliferaon to improve wound healing. Black tamanu oil has been reported for its wound
healing and anmicrobial acvity against skin bacteria. Thus, this experiment studied the wound
healing and anmicrobial acvity of biocellulose and black tamanu oil through an in-vivo study using
diabec mice and non-diabec rabbits as animal models. The treatment groups included BC-black
tamanu oil, BC-only, and gauze (control). The wound healing acvity was studied by measuring the
wound reducon from excisional wound. BC-black tamanu oil showed stascally significant
improvement in wound reducon in non-diabec rabbits, while it did not significantly improve
diabec mice wounds compared to BC-only and gauze groups. The microbial study was studied using
Miles-Misra method and the results showed BC-black tamanu oil did not have notable anmicrobial
acvity compared to BC-only and gauze. Histology study was done using Hematoxylin-Eosin staining
and it showed that BC-black tamanu oil treatment accelerates granulaon ssue formaon, collagen
deposion, and re-epithelializaon in both diabec and non-diabec wounds. Thus, it is concluded
from this study, BC-black tamanu oil may be beneficial for accelerang wound healing by improving
the inflammaon phase and promotes them into a proliferave phase.

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Availability

Detail Information

Series Title

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Call Number

PHA 23-014

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

biocellulose
Black Tamanu Oil
In-Vivo
Wound healing study
Microbial study

Specific Detail Info

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Statement of Responsibility

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