Thesis

Understanding the Roles of on Cell Surface in Migration and Invasion of Cells

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Breast cancer is one of the most commonly found cancer cases worldwide with a relatively high
mortality rate. It was found to be highly correlated with metastasis properties of cancer cells that
facilitate their rapid invasion and motility. The reduction of disulfide bonds into active thiol functional
groups was previously found to be able to reduce the migration and invasion of other cancer types.
Therefore, this study aimed to investigate the effect of active thiol group reduction on the migration
and invasion of two human breast cancer cell lines (MCF-7 and MDA-MB-231). TCEP was used to
induce the expression of active thiol functional groups on the surface of the cells in order to enhance
the cell-ECM binding and adhesion, resulting in reduced migration and invasion. This study found that
TCEP treatment did not significantly reduce the migration (p > 0.05) of both MCF-7 and MDA-MB-231
cells through the horizontal migration (wound healing assay). However, the result of vertical
migration (transwell migration assay) significantly reduced (p
≤0.01) the migration of MDA-MB-231 cells. The invasion of both cells towards the matrix aligns with the wound healing assay, where it was
non-significantly reduced after the TCEP treatment. Lastly, changes in cell migration and
invasion-related gene expression of the TCEP-treated groups were also found to be non-significantly
(p > 0.05) different from the control groups. However, the non-significant results obtained in this
research study underscore the need for further investigation and consideration of alternative factors
that may influence the outcome of the results.

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Availability

Detail Information

Series Title

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Call Number

PHA 23-013

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

breast cancer
metastasis

Specific Detail Info

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Statement of Responsibility

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