Thesis

In Vivo Wound healing and Antibacterial Activity Study of Black Tamanu Keratin Biocellulose on Rabbit Model

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Wound healing is a process that naturally takes place when the body is injured, comprising four
phases: hemostasis, inflammaon, proliferaon, and remodeling. Proper wound care is crucial to
prevent infecon and complicaons that can impact the paent's quality of life, and to accelerate the
healing process. Biocellulose (BC), a bacterial-derived biopolymer, was used as a wound dressing due
to its beneficial properes and moisture. Hair-derived keran promotes ssue remodeling and
epithelializaon, accelerang the process. Combining keran biocellulose (kBc) with black tamanu
(BT) oil, known for its anbacterial and an-inflammatory properes, enhances healing. Rabbits were
ulized to evaluate the wound healing and anbacterial properes of black tamanu keran
biocellulose (BT kBc). This study aimed to assess the wound healing and anbacterial acvity of BT
kBc through an in vivo study on rabbit model, using excisional wound model; and total viable count
and gram staining, respecvely. The healing acvity study showed that BT kBc and kBc treated
wounds was closed faster compared to gauze. On day 6 aer wound creaon, BT kBc and kBc wounds
had % wound closure of 42.5% and 44.9%, respecvely, while the control was 16.2%. Histology study
using hematoxylin and eosin stain revealed that kBc and BT kBc wounds were in the proliferaon
phase, while the control wound was in the inflammatory phase. The anbacterial study showed that
BT and kBc exerted anbacterial acvity towards gram-posive bacteria, but the bacterial count could
not be determined.

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Availability

Detail Information

Series Title

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Call Number

PHA 23-012

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Wound healing
biocellulose
Keran
Rabbit
Black Tamanu

Specific Detail Info

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Statement of Responsibility

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