Thesis

Study of Teratogenic Effect of Herbal Medicines Marketed in Indonesia Using Zebrafish Embryo

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With the rising popularity, herbal medicine has increasing consumption with a minimum 80%
worldwide population or use it as their primary medicine depending on their culture and location.
The usage of herbal medicine during pregnancy in Indonesia was reported to be 60% in 2020 due to
an attempt to alleviate common side effects of pregnancy. However, herbal medicine has not been
regulated enough in some countries, thus the teratogenic effect remains unknown. This study focuses
on using zebrafish embryos to study the teratogenic effect of marketed herbal medicines in
Indonesia. Zebrafish is a good candidate with its high genetic similarity to humans, high fertility, short
lifecycle, and transparency for ease of observation. Furthermore, the LC50 value for teratogenicity
characterization was also evaluated. The Zebrafish FET study from OECD was used as the main
method in this study to see the morphological deformity. The results showed that all herbal medicine
tested showed teratogenicity through morphological changes, with the main teratogenicity being
pericardial edema, yolk sac edema, tail bent, and coagulation with teratogenic index value of 1.55,
1.18, and 1.61, respectively. Though, LC50 of drug 1 and drug 2, with concentration of 0.5431 mg/mL,
1.456 mg/mL, respectively, are considered as safe while drug 3 with concentration 0.0946 mg/mL are
considered as harmful according to OECD characterization.

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Availability

Detail Information

Series Title

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Call Number

PHA 23-007

Publisher

i3L, Jakarta : i3L, Jakarta., 2023

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Zebrafish teratogenicity study
Herbal medicine teratogenicity
Fish Embryotoxicity Test

Specific Detail Info

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Statement of Responsibility

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